Neuroprotective effect of Nevirapine on cerebral ischemic stroke by middle cerebral artery occlusion in wistar rats

CH Maneesha Ram, Nazia Begum, Naveen Pathakala, Dr. Vasudha Bakshi


Objective: To evaluate the neuroprotective effect of Nevirapine on cerebral ischemia stroke by middle cerebral artery occlusion in wistar rats.

Methods:   The rats were pre and post treated with Nevirapine (NVP) at selective doses (5, 10 mg/kg/g, p.o) for a period of 14 days followed by middle cerebral artery occlusion (MCAO). Neurobehavioral changes were evaluated by using Y-maze and open field habituation. Biochemical markers such as acetyl cholinesterase (AChE), glutamate, differential leukocyte count (DLC), lactate dehydrogenase (LDH), antioxidants such as superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase were estimated.Results: Obtain results revealed that 14 days of treatment with NVP was effective in averting neurotoxicity. NVP treatment significantly reduced AChE, glutamate, DLC, LDH and elevated levels of antioxidant parameters such as SOD, catalase and GPX..Conclusion: These results clearly revealed that Nevirapine exhibited cognitive improvement which is related to its antioxidant and neuroprotective activity. Further studies are suggested to evaluate molecular mechanism of involved in neuroprotection.



Neuroprotective effect; Nevirapine; cerebral ischemic stroke; middle cerebral artery; occlusion;

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Di Tullio MR, Sacco RL, Gersony D, Nayak H, Weslow RG, Kargman DE, and Homma S (1996). Aortic atheromas and acute ischemic stroke: atransesophageal echocardiographic study in an ethnically mixed population. Neurology. 46, 1560-1566.

Enting RH, Hoetelmans RMW and Lange JMA (1998). Antiretroviral drugs and the Central Nervous System. AIDS .12, 1941-1955.

Gibbs JE, Gaffen Z, Thomas SA (2006). Nevirapine uptake into the central nervous system of the Guinea pig: an in situ brain perfusion study. The Journal of pharmacology and experimental therapeutics. 317, 746–751.

Goth L (1991). A simple method for determination of serum catalase activity and revision of reference range. International journal of clinical chemistry.196, 143–152.

Kilby JM, Eron JJ (2003) .Novel therapies based on mechanisms of HIV-1 cell entry. New England journal of Medicine. 348(22), 2228-38.

Koladiya RU, Jaggi AS, Singh N, Sharma BK (2008). Ameliorative role of atorvastatin and pitavastatin in L-methion induced vascular dementia in rats. BMC Pharmacol. 8, 1–12.

Krause GS, White BC, Aust SD, Nayini NR, Kumar K (1988). Brain cell death following ischemia and reperfusion: a proposed biochemical sequence. The open critical care medicine journal. 16, 714-726.

Lawrence RA, Burk RF (1976). Glutathione peroxide activity in Selenium deficient rat liver . Biochemical and biophysical research communications. 71, 952-958.

Lisa Mosconi (2013). Glucose metabolism in normal aging and Alzheimer’s disease: methodological and physiological considerations for PET studies. Clin Transl Imaging. 1(4). doi:10.1007/s40336-013-0026-y.

Lorentz K, Klauke R, Schmidt E (1993). Recommendations for the determination of the catalytic concentration of Lactate dehydrogenase at 37oC. Eur J Clin Chem Clin Biochem. 31: 897-899.

Marklund S, Marklund G (1974). Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European journal of biochemistry.47, 469–474.

Maria R R, Ivan I, Maria CBR, Jose AZ, Daniela B, Amelia TII (2005). Effect of Lyophilised Vaccinium Berries on memory,anxiety and locomotion n adult rats. Journal of experimental pharmacology. 52, 157-162.

Marta Wietrzych, Hamid Meziane, Anne Sutter, Norbert Ghyselinck, Paul F Chambon, Wojciech Krezel (2005). Working memory deficits in retinoid X receptor Y-deficient mice. Learning Memory. 12(3): 318-326.

Nair D, Kumar KR, Srikumar BN, Raju TR, Rao SB, (2004). Estimation of neurotransmitters in the brain by chromatographic methods. Brain and behavior. 134–141.

Natalia Sestakova, Angelika Puserova, Michal Kluknavsky, Iveta Bernatova (2013). Determination of motor activity and anxiety-related behavior in rodents: methodological aspects and role of nitric oxide. Interdisciplinary toxicology. 6(3), 126-135.

Reddy DS (1997). Assessment of notropic and amnestic activity of centrally acting agents. Indian journal of pharmacology. 29, 208–221.

R Shanmuga Sundaram, L Gowtham, Bhabani S. Nayak (2012). The role of Excitatory Neurotransmitter Glutamate in brain physiology and pathology. Asian Journal of pharmaceutical and clinical research. 5(2), 1-7.

Satyabrata kar, Stephen PM Slowikowski, David Westaway, Howard TJ Mount (2004). Interactions between Beta-amyloid and central cholinergic neurons: Implications for Alzheimer’s disease. Journal of Psychiatry &Neuroscience. 29(6), 427-441.

Siesjo BK, Siesjo P (1996). Mechanisms of secondary brain injury. European journal of anaesthesiology 13, 247-268.

Tashima KT, Caliendo AM, Ahmad M, Gormley JM, Fiske WD, Brennan JM, Flanigan TP. (1999). Cerebrospinal fluid human immunodeficiency virus type 1 (HIV-1) suppression and efavirenz drug concentrations in HIV-1-infected patients receiving combination therapy. Journal of infectious diseases. 180, 862–864.

Treisman GJ, Kaplin AI (2002). Neurologic and psychiatric complications of antiretroviral agents. AIDS. 16, 1201–1215.

Von Giesen HJ, Koller H, Theisen A, Arendt G (2002). Therapeutic effects of nonnucleoside reverse transcriptase inhibitors on the central nervous system in HIV-1-infected patients.Journal of acquired immune deficiency syndromes. 29, 363–367.

Wynn HE, Brundage RC, Fletcher CV (2002) Clinical implications of CNS penetration of antiretroviral drugs. CNS Drugs. 16, 595–609.

Zhi-Guo Zhang, Xin Sun, Quing-Zhu Zhang and Hua Yang (2013).Neuroprotective effect of ultra-low-molecular-weight heparin on Cerebral Ischemia/Reperfusion injury in rats: involvement of apoptosis, inflammarory reaction and energy metabolism. Int J Mol Sci. 14(1), 1932-1939.



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