Seed Germination Inhibitors: Molecular and Phytochemical Aspects

Saptarshi Samajdar, Souvik Mukherjee, Partha Pratim Das


Two major stages of seed development are dormancy and germination which finally promotes the growth of a plant. Some internal and external factors such as hormonal, genetic, chromatin development and environmental factors which maintain the seed dormancy with passing time and other suitable factors, these dormancy promoters are gradually decreased causing release of dormancy and promoting germination by the mechanisms of ROS in plant signalling, cell elongation and reverse mobilization. But dormancy has some benefits in protecting the seed from extreme condition even after natural disaster as well as serving as food for predators in order to maintain balance of nature. So, dormancy can be inhibited by some phytochemical components like terpenoids, polyphenoliic compounds, flavonoids, alkaloids and glycosides by the mechanism of inhibiting water uptake system III & II, surface sterilization, reverse mobilization, cell elongation etc.


Dormancy; Germination inhibitors; Polyphenols; essential oils; glycosides; alkaloids.

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Achard P, Gong F, Cheminant S, Alioua M, Hedden P & Genschik P (2008). The cold-inducible CBF1 factor–dependent signaling pathway modulates the accumulation of the growth-repressing DELLA proteins via its effect on gibberellin metabolism. The Plant Cell. 20(8): 2117-2129.

Ali-Rachedi S, Bouinot D, Wagner M.-H, Bonnet M, Sotta B, Grappin P & Jullien M (2004). Changes in endogenous abscisic acid levels during dormancy release and maintenance of mature seeds: studies with the Cape Verde Islands ecotype, the dormant model of Arabidopsis thaliana. Planta. 219(3):479-488.

Barrero JM, Millar AA, Griffiths J, Czechowski T, Scheible WR, Udvardi M (2010). Gene expression profiling identifies two regulatory genes controlling dormancy and ABA sensitivity in Arabidopsis seeds. The Plant Journal. 61(4):611-622.

Bassel GW, Lan H, Glaab E, Gibbs DJ, Gerjets T, Krasnogor N, Provart, NJ (2011). Genome-wide network model capturing seed germination reveals coordinated regulation of plant cellular phase transitions. Proceedings of the National Academy of Sciences. 108(23):9709-9714.

Bentsink L, Hanson J, Hanhart CJ, Blankestijn-de Vries H, Coltrane C, Keizer P, Reymond M (2010). Natural variation for seed dormancy in Arabidopsis is regulated by additive genetic and molecular pathways. Proceedings of the National Academy of Sciences. 107(9):4264-4269.

Bentsink L, Jowett J, Hanhart CJ & Koornneef M (2006). Cloning of DOG1, a quantitative trait locus controlling seed dormancy in Arabidopsis. Proceedings of the National Academy of Sciences, 103(45):17042-17047.

Bewley JD (1997). Seed germination and dormancy. The Plant Cell. 9(7):1055.

Bewley JD & Black M (1994). Seeds (pp. 1-33): Springer.

Bradford KJ (1996). Population-based models describing seed dormancy behaviour: implications for experimental design and interpretation. Plant dormancy: physiology, biochemistry and molecular biology. Wallingford, UK: CAB International. 313-339.

Chattha F, Munawar M, Ashraf M, Kousar S & Nisa M (2015). Plant growth regulating activities of coumarin-3-acetic acid derivatives. Allelopathy Journal. 36(2).

Chiang GC, Barua D, Kramer EM, Amasino RM & Donohue K. (2009). Major flowering time gene, FLOWERING LOCUS C, regulates seed germination in Arabidopsis thaliana. Proceedings of the National Academy of Sciences. 106(28):11661-11666.

Dastan D, Salehi P, Ghanati F, Gohari A R, Maroofi H & Alnajar N (2014). Phytotoxicity and cytotoxicity of disesquiterpene and sesquiterpene coumarins from Ferula pseudalliacea. Industrial Crops and Products. 55:43-48.

Dixon RA, Xie DY & Sharma SB. (2005). Proanthocyanidins–a final frontier in flavonoid research. New Phytologist. 165(1):9-28.

El-Sadek A, Balah M, Romani A, Ieri F, Vignolini P, Salem E, Virtuosi I. (2017). Allelopathic potential of quinoa (Chenopodium quinoa willd.) genotypes on the germination and initial development of some weeds and crops. Egyptian J. Desert Res. 67(1):25-45.

Finch‐Savage WE & Leubner‐Metzger G (2006). Seed dormancy and the control of germination. New phytologist. 171(3):501-523.

Footitt S, Douterelo-Soler I, Clay H & Finch-Savage WE (2011). Dormancy cycling in Arabidopsis seeds is controlled by seasonally distinct hormone-signaling pathways. Proceedings of the National Academy of Sciences. 108(50):20236-20241.

Foyer CH, Lam HM, Nguyen HT, Siddique KH, Varshney RK, Colmer TD, Hodgson JM (2016). Neglecting legumes has compromised human health and sustainable food production. Nature Plants. 2:16112.

Gascó G, Cely P, Paz-Ferreiro J, Plaza C & Méndez A (2016). Relation between biochar properties and effects on seed germination and plant development. Biological Agriculture & Horticulture. 32(4):237-247.

Gawlik-Dziki U, Dziki D, Świeca M & Nowak R (2017). Mechanism of action and interactions between xanthine oxidase inhibitors derived from natural sources of chlorogenic and ferulic acids. Food chemistry. 225:138-145.

Graeber K, Linkies A, Müller K, Wunchova A, Rott A & Leubner-Metzger G (2010). Cross-species approaches to seed dormancy and germination: conservation and biodiversity of ABA-regulated mechanisms and the Brassicaceae DOG1 genes. Plant molecular biology. 73(1-2):67-87.

Grichi A, Nasr Z & Khouja ML (2016). Phytotoxic Effects of Essential Oil from Eucalyptus lehmanii against Weeds and its Possible Use as a Bioherbicide. Bulletin of Environment, Pharmacology and Life Sciences. 5:17-23.

Harborne JB & Williams CA. (2000). Advances in flavonoid research since 1992. Phytochemistry. 55(6):481-504.

Ho GTT, Kase ET, Wangensteen H & Barsett H (2017). Phenolic Elderberry Extracts, Anthocyanins, Procyanidins, and Metabolites Influence Glucose and Fatty Acid Uptake in Human Skeletal Muscle Cells. Journal of Agricultural and Food Chemistry. 65(13):2677-2685.

Holdsworth MJ, Bentsink L & Soppe WJ (2008). Molecular networks regulating Arabidopsis seed maturation, after‐ripening, dormancy and germination. New phytologist. 179(1):33-54.

Hossain MA, Munemasa S, Uraji M, Nakamura Y, Mori IC & Murata Y (2011). Involvement of endogenous abscisic acid in methyl jasmonate-induced stomatal closure in Arabidopsis. Plant Physiology. 156(1):430-438.

Huang X, Zhang X, Gong Z, Yang, S & Shi Y (2017). ABI4 represses the expression of type‐A ARRs to inhibit seed germination in Arabidopsis. The Plant Journal. 89(2):354-365.

Huang Z, Footitt S, Tang A & Finch‐Savage W (2018). Predicted global warming scenarios impact on the mother plant to alter seed dormancy and germination behaviour in Arabidopsis. Plant, Cell & Environment. 41(1):187-197.

Kouris-Blazos A & Belski R (2016). Health benefits of legumes and pulses with a focus on Australian sweet lupins. Asia Pacific journal of clinical nutrition. 25(1):1-17.

Kuromori T, Miyaji T, Yabuuchi H, Shimizu H, Sugimoto E, Kamiya A, Shinozaki K (2010). ABC transporter AtABCG25 is involved in abscisic acid transport and responses. Proceedings of the National Academy of Sciences, 107(5):2361-2366.

Li W, Yamaguchi S, Khan MA, An P, Liu X & Tran L.-S P (2016). Roles of gibberellins and abscisic acid in regulating germination of Suaeda salsa dimorphic seeds under salt stress. Frontiers in plant science. 6:1235.

Linkies A & Leubner-Metzger G (2012). Beyond gibberellins and abscisic acid: how ethylene and jasmonates control seed germination. Plant cell reports. 31(2):253-270.

Linkies A, Müller K, Morris K, Turečková V, Wenk M, Cadman CS, Finch-Savage WE (2009). Ethylene interacts with abscisic acid to regulate endosperm rupture during germination: a comparative approach using Lepidium sativum and Arabidopsis thaliana. The Plant Cell. 21(12):3803-3822.

Liu Y, Koornneef M & Soppe WJ (2007). The absence of histone H2B monoubiquitination in the Arabidopsis hub1 (rdo4) mutant reveals a role for chromatin remodeling in seed dormancy. The Plant Cell. 19(2):433-444.

Mahajan P, Batish DR, Singh HP & Kohli RK (2016). β-Pinene partially ameliorates Cr (VI)-inhibited growth and biochemical changes in emerging seedlings. Plant growth regulation, 79(2):243-249.

Manz B, Müller K, Kucera B, Volke F & Leubner-Metzger G (2005). Water uptake and distribution in germinating tobacco seeds investigated in vivo by nuclear magnetic resonance imaging. Plant Physiology. 138(3):1538-1551.

Marles MS, Ray H & Gruber MY (2003). New perspectives on proanthocyanidin biochemistry and molecular regulation. Phytochemistry. 64(2):367-383.

Mendoza-Sánchez M, Guevara-González RG, Castaño-Tostado E, Mercado-Silva EM, Acosta-Gallegos JA, Rocha-Guzmán NE & Reynoso-Camacho R (2016). Effect of chemical stress on germination of cv Dalia bean (Phaseolus vularis L.) as an alternative to increase antioxidant and nutraceutical compounds in sprouts. Food chemistry. 212:128-137.

Mohammed NMF (2016). Fragrant secondary Metabolites of Acacia seyal, Combretum hartmannianum and Terminalia laxiflora Fungal Fermented Wood Extracts Used in Sudanese Cosmetics. Sudan University of Science and Technology.

Nandagopalan V (2017). Effect of heat treatment on germination, seedling growth and some biochemical parameters of dry seeds of black gram. International Journal of Pharmaceutical and Phytopharmacological Research. 1(4):194-202.

Narro‐Diego L, López‐González L, Jarillo JA & Piñeiro M (2017). The PHD‐containing protein EARLY BOLTING IN SHORT DAYS regulates seed dormancy in Arabidopsis. Plant, Cell & Environment. 40(10):2393-2405.

Née G, Kramer K, Nakabayashi K, Yuan B, Xiang Y, Miatton E, Soppe WJ (2017). DELAY OF GERMINATION1 requires PP2C phosphatases of the ABA signalling pathway to control seed dormancy. Nature Communications. 8(1):72.

Nelson SK & Steber CM (2017). Transcriptional mechanisms associated with seed dormancy and dormancy loss in the gibberellin-insensitive sly1-2 mutant of Arabidopsis thaliana. PloS one. 12(6):e0179143.

Oracz K, Bouteau HEM, Farrant JM, Cooper K, Belghazi M, Job C, Bailly C (2007). ROS production and protein oxidation as a novel mechanism for seed dormancy alleviation. The Plant Journal. 50(3):452-465.

Park YS, Beaulieu J & Bousquet J (2016). Multi-varietal forestry integrating genomic selection and somatic embryogenesis. Vegetative Propagation of Forest Trees. Seoul: National Institute of Forest Science (NiFos). 302-322.

Peña‐Castro JM, van Zanten M, Lee SC, Patel MR, Voesenek LA, Fukao T & Bailey‐Serres, J (2011). Expression of rice SUB1A and SUB1C transcription factors in Arabidopsis uncovers flowering inhibition as a submergence tolerance mechanism. The Plant Journal. 67(3):434-446.

Penfield S, Josse E.-M & Halliday KJ (2010). A role for an alternative splice variant of PIF6 in the control of Arabidopsis primary seed dormancy. Plant molecular biology. 73(1-2):89-95.

Penfield S, Josse EM, Kannangara R, Gilday AD, Halliday KJ & Graham IA (2005). Cold and light control seed germination through the bHLH transcription factor SPATULA. Current Biology. 15(22):1998-2006.

Pošta M, Papenfus HB, Light ME, Beier P & Van Staden J (2017). Structure–activity relationships of N-and S-analogs of the seed germination inhibitor (3, 4, 5-trimethylfuran-2 (5H)-one) for mode of action elucidation. Plant Growth Regulation. 82(1):47-53.

Probert RJ (2000). The role of temperature in the regulation of seed dormancy and germination. Seeds: the ecology of regeneration in plant communities. 2:261-292.

Radchuk R, Conrad U, Saalbach I, Giersberg M, Emery R, Küster H, Weber H (2010). Abscisic acid deficiency of developing pea embryos achieved by immunomodulation attenuates developmental phase transition and storage metabolism. The Plant Journal. 64(5):715-730.

Roudier F, Ahmed I, Bérard C, Sarazin A, Mary‐Huard T, Cortijo S, Al‐Shikhley L (2011). Integrative epigenomic mapping defines four main chromatin states in Arabidopsis. The EMBO journal. 30(10):1928-1938.

Rozmer Z & Perjési P (2016). Naturally occurring chalcones and their biological activities. Phytochemistry reviews. 15(1):87-120.

Saleh AM & Madany M (2015). Coumarin pretreatment alleviates salinity stress in wheat seedlings. Plant Physiology and Biochemistry. 88:27-35.

Scheel LD (2016). The biological action of the coumarins. Microbial toxins. 8:47-66.

Shao C, Liu J, Zhang S & Zhang Y (2016). Bioassay of endogenous germination inhibitors in Trillium kamtschaticum seed. Seed Science and Technology. 44(1):224-232.

Singh P, Dave A, Vaistij FE, Worrall D, Holroyd GH, Wells JG, Roberts MR (2017). Jasmonic acid‐dependent regulation of seed dormancy following maternal herbivory in Arabidopsis. New phytologist. 214(4):1702-1711.

Smith SA & Donoghue MJ (2008). Rates of molecular evolution are linked to life history in flowering plants. Science. 322(5898):86-89.

Weitbrecht K, Müller K & Leubner-Metzger G (2011). First off the mark: early seed germination. Journal of experimental botany. 62(10):3289-3309.

Wojtyla Ł, Garnczarska, M., Zalewski, T., Bednarski, W., Ratajczak, L., & Jurga, S. (2006). A comparative study of water distribution, free radical production and activation of antioxidative metabolism in germinating pea seeds. Journal of plant physiology, 163(12), 1207-1220.

Wolkovich EM, Cook BI, Allen JM, Crimmins TM, Betancourt JL, Travers SE, Kraft, NJ (2012). Warming experiments underpredict plant phenological responses to climate change. Nature. 485(7399):494.

Wu CX, Zhao GQ, Liu DL, Liu SJ, Gun XX & Tang Q (2016). Discovery and Weed Inhibition Effects of Coumarin as the Predominant Allelochemical of Yellow Sweetclover (Melilotus officinalis). International Journal of Agriculture & Biology. 18(1).

Xu Q, Truong TT, Barrero JM, Jacobsen JV, Hocart CH & Gubler F (2016). A role for jasmonates in the release of dormancy by cold stratification in wheat. Journal of experimental botany. 67(11):3497-3508.

Yamaguchi S (2008). Gibberellin metabolism and its regulation. Annu. Rev. Plant Biol. 59:225-251.

Yao DD, Wang JY, Zhou Q, Tang Q, Zhao GQ & Wu CX (2017). Effect of coumarin on Italian ryegrass seed germination and seedling growth. Acta Prataculturae Sinica. 2:015.

Zapata L, Ding J, Willing EM, Hartwig B, Bezdan D, Jiao WB, Ossowski S (2016). Chromosome-level assembly of Arabidopsis thaliana Ler reveals the extent of translocation and inversion polymorphisms. Proceedings of the National Academy of Sciences. 113(28):E4052-E4060.



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