RP-HPLC Method for the Simultaneous Estimation of Ampicillin and Cloxacillin in Capsule Dosage Form

Shireesha Battula, Anusha Shivaraj


Combination  formulation  of   ampicillin  and  cloxacillin  sodium is β-lactam antibiotic. They are amino  penicillin–isoxazyl penicillin combination drugs. In HPLC method development was carried out with acetonitrile: phosphate buffer pH 5 (35:65), hypersil C18, 250X4.6mm, 5μm column, UV detector at 254nm and flow rate of 1ml/min. Ampicillin retention time is 3.130min and cloxacillin retention  time  is  7.907min.  In  capsule  brand  no.:1. ampicillin  content is found  to  be  250.29mg i.e. 100.1% and  cloxacillin  content  is  found  to be 252.01mg. i.e. 102.75%. Validation  results  obtained   are   satisifactory  for accuracy, precision, limit of detection, limit of quantification, linearity, system suitability, ruggedness and robustness parameters.


RP-HPLC; estimation; ampicillin; cloxacillin; simultaneous; capsule dosage form;

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DOI: http://dx.doi.org/10.21477/ijapsr.v2i03.8255


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