Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Metformin and Glipizide in Tablet Dosage Form

K. Ganesh, G. Nikitha, D. Sireesha, B. Vasudha

Abstract


A simple, rapid and precise spectrophotometric method has been developed for simultaneous estimation of Metformin and Glipizide. The method involved estimation of Metformin and Glipizide by simultaneous equation at 272nm and 232nm respectively in their solution in water. This method was validated with respect to linearity, accuracy, precision, LOD and LOQ. Beer’s law obeyed in the concentration range of 5-25μg/ml and 20-50μg/ml for Metformin and Glipizide respectively with the correlation coefficient of above 0.99. Limit of detection and quantification values were determined to be 0.214μg/ml and 0.649μg/ml for Metformin and 0.608μg/ml and 1.854μg/ml for Glipizide respectively. Mean recovery of Metformin and Glipizide were found to be in the range of 98-102% signifies the accuracy of the method. The method was found to be precise as %RSD was less than 2.

Keywords


Development; Validation; UV Spectrophotometric Method; Simultaneous; Estimation;Metformin; Glipizide; Tablet Dosage Form

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References


D.Triveni, G.V.S Kumar, S.B Puranik, N.Sateesh Kumar, K.A Sridhar (2012). Development and validation of RP-HPLC method for simultaneous estimation of Glipizide and Metformin in bulk drugs and pharmaceutical dosage form. International Research Journal of Pharmacy. 3(9), 260-263. 2. K.S. Lakshmi, T. Rajesh, S. Sharma, S. Lakshmi (2009). Development and validation of liquid chromatographic and UV derivative spectrophotometric methods for the determination of Metformin, Pioglitazone and Glimepiride in pharmaceutical formulations. Der Pharma Chemica. 1(1), 238-246.

L Adhikari, S Jagadev , S Sahoo , P.N Murthy ,U.S Mishra (2012). Devlopement and Validation of UV-Visible Spectrophotometric Method for Simultaneous Determination of pioglitazone Hydrochloride, Metformin Hydrochloride and Glipizide in its Bulk and Pharmaceutical Dosage Form (Tablet). International Journal of Chem Tech Research. 4(2), 625-630.

L Bhaskar Reddy, P. Raveendra Reddy, Useni Reddy Mallu and L Maheshwara Reddy (2011). Novel RP-HPLC Method for Metformin HCL, Glipizide and Repaglinide pharmaceutical Drug Products. International Journal of Research and Reviews in Pharmacy and Applied Sciences. 1(3), 131-139.

Li Gong, Srijib Goswami, Kathleen M Giacomini, Russ B Altman, Teri E Klein (2012). Merformin Pathways: Pharmacokinetics and Pharmacodynamics. Pharmacogenet Genomics. 22(11), 820-827.

Rashmi Ranjan Sarangi, Satya Narayana Panda, Susanta Kumar Panda, Kanhu Charana Sahu (2011). Simultaneous UV-Spectrophotometric Estimation of Glipizide and Metformin in Bulk and Its Dosage Form. International Journal of Pharmaceutical & Biological Archives. 2(4), 1137-1145

Ripudaman S Hondal, Martin Krssak, Sylvie Dufour, Didier Laurent, Vincent Lebon, Visvanathan Chandramooli, Silvio E Inzucchi, William C Schumam, Kitt F Petersen, Bernard R Landau and Gerald Shulman (2000). Mechanism by which Metformin Reduces Glucose Production in Type 2 Diabetes. Diabetes. 49(12), 2063-2069.

Sabyasachi Mandal1 Sabyasachi Mandal1 , Dr. Anirbandeep Bose , Dr. Gurubasavaraja Swamy, Shree Narayan Pandit , Dr. Divakar Goli (2015). Stability indicating assay method by HPLC for simultaneous estimation of metformin and glipizide in bulk and pharmaceutical dosage form. World journal of pharmacy and pharmaceutical sciences. 4(6), 1410-1431.

Sri Lakshmi D, Jane T Jacob, Srinivas D, Satyanarayana D (2015). Simultaneous estimation of metformin and glipizide by RP-HPLC and its validation. World journal of pharmacy and pharmaceutical sciences. 4(9), 740-750.

T RamanjiReddy, D Dachinamoorthi, K B Chandrasekhar (2011). Importance of Merformin with Repaglinide in the Treatment of Type II Diabetes Mellitus: A Decadal Review. Asian Journal of Pharmaceutical and Clinical Research. 5(1), 1-4.




DOI: http://dx.doi.org/10.21477/ijapsr.v1i2.10176

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